Harmful Algal Blooms Monitoring by Metabarcoding Analysis | Protocol Preview

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A Standardized Procedure for Monitoring Harmful Algal Blooms in Chile by Metabarcoding Analysis - a 2 minute Preview of the Experimental Protocol

Kyoko Yarimizu, So Fujiyoshi, Mikihiko Kawai, Jacquelinne J. Acuña, Joaquin-Ignacio Rilling, Marco Campos, Jonnathan Vilugrón, Henry Cameron, Karen Vergara, Gonzalo Gajardo, Oscar Espinoza-González, Leonardo Guzmán, Satoshi Nagai, Carlos Riquelme, Milko A. Jorquera, Fumito Maruyama
Hiroshima University, Office of Research and Academia-Government-Community Collaboration; Kyoto University, Graduate School of Human and Environmental Studies; Universidad de La Frontera, Scientific and Biotechnological Bioresource Nucleus; Instituto de Fomento Pesquero, Centro de Estudios de Algas Nocivas; Universidad de Antofagasta, Ciencias del Mar y Recursos Biologicos; Universidad de Los Lagos, Departamento de Ciencias Biológicas y Biodiversidad; Fisheries Resources Institute, Japan Fisheries Research and Education Agency;

This protocol introduces steps of metabarcoding analysis, targeting 16S rRNA and 18S rRNA genes, for monitoring harmful algal blooms and their associated microbiome in seawater samples. It is a powerful molecular-based tool but requires several procedures, which are visually explained here step-by-step.

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