Histone Post-Translational Modifications By Bottom-Up Mass Spectrometry l Protocol Preview

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Complete Workflow for Analysis of Histone Post-translational Modifications Using Bottom-up Mass Spectrometry: From Histone Extraction to Data Analysis - a 2 minute Preview of the Experimental Protocol

Simone Sidoli, Natarajan V. Bhanu, Kelly R. Karch, Xiaoshi Wang, Benjamin A. Garcia
University of Pennsylvania, Epigenetics Program, Department of Biochemistry and Biophysics, Perelman School of Medicine;

This protocol outlines a fully integrated workflow for characterizing histone post-translational modifications using mass spectrometry (MS). The workflow includes histone purification from cell cultures or tissues, histone derivatization and digestion, MS analysis using nano-flow liquid chromatography and instructions for data analysis. The protocol is designed for completion within 2 - 3 days.

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