Heat-Shock Transformation Protocol (for Bacteria)

Hey Friends,

the Heat-Shock Transformation protocol for chemically competent cells after Calicum Chloride (CaCl2) treatment is a method to introduce genes into bacteria. A short heat shock makes it able for the cells to take up plasmid DNA from the environment.

Transformation, Conjugation and Transduction:
   • Transformation, Transduction and Conj...  


References:
www.ncbi.nlm.nih.gov/pmc/articles/PMC5681917/
www.ncbi.nlm.nih.gov/pmc/articles/PMC5326489/


Thanks
Henrik


Time Codes:
0:00 Introduction
0:59 Transformation in the lab
2:57 Plating bacteria
3:26 Outro